QuicKey Pro Mouse IL-18 (Interleukin 18) ELISA Kit

QuicKey Pro Mouse IL-18 (Interleukin 18) ELISA Kit

• Abbreviation: IL-18
• Synonyms: IL18; IGIF; IL-1g; IL-1F4; Interferon-gamma-inducing factor
• CAS Number: 7732-18-5
• UniProt: P70380
• Cross Reactivity: This kit recognizes IL-18 in samples.No significant cross-reactivity or interference between IL-18 and analogues was observed.
• Target: Mouse
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cancer; Metabolism; Immunology
• Detection Method: Colorimetric method; ELISA; Sandwich
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-18 are added to the micro ELISA plate wells. Mouse IL-18 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-18.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. The concentration of Mouse IL-18 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.