Pyronin Y

• UNSPSC Description:

  Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells[1][2][5].

• Target Antigen:

  DNA Stain

• Type:

  Dye Reagents

• Related Pathways:

  Cell Cycle/DNA Damage

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/Pyronin_Y.html

• Purity:

  82.2

• Solubility:

  DMSO : 25 mg/mL (ultrasonic)|H2O : 4 mg/mL (ultrasonic)

• Smiles:

  CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C=C2C=C1)C.[Cl-]

• Molecular Weight:

  302.80

• References & Citations:

  [1]Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35.|[2]Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20.|[3]Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6.|[4]Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303.|[5]Laura M Andrews, et al. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed Opt Express. 2013 Jan 1;4(1):171-7.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, sealed storage, away from moisture and light)

• Clinical Information:

  No Development Reported

• CAS Number:

  92-32-0