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SPAG16 Antibody

CAT: 0013-GTR18126464Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126464Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SPAG16 Antibody
Product Name Alternative
SPAG16, sperm associated antigen 16, DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29, WD repeat domain 29, sperm-associated WD repeat protein
UniProt
Q8N0X2
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KLHYASYEPTIR
Target
SPAG16
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 22kDa in Human Testis lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 70.8kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_078808.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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