Products for Research Use Only

Fut1 Antibody

CAT: 0013-GTR18126383Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126383Size:0.1 mg
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Description
Fut1 Antibody
Product Name Alternative
Fut1, fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group), H, HH, HSC, 2-alpha-L-fucosyltransferase, GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1, alpha (1,2) fucosyltransferase, alpha (1,2) FT 1, blood group H alpha 2-fucosyltransferase, fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase), galactoside 2-alpha-L-fucosyltransferase 1
UniProt
O09160
Immunogen
The immunogen for this antibody is: C-QLNGRQAFIQPEMH
Target
Fut1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 30kDa in Mouse and Rat Brain lysates after 0.5μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.4kDa according to NP_032077.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Mouse, Porcine, Rat
Tested Applications
ELISA
NCBI Accession Number
NP_032077.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · O09160

Galactoside alpha-(1,2)-fucosyltransferase 1

FUT1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O09160
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Fut1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
P97327
Protein keywords

PTM

Glycoprotein

Molecular function

GlycosyltransferaseTransferase

Cellular component

Golgi apparatusMembrane

Biological process

Lipid metabolism

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix