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KEAP1 Antibody

CAT: 0013-GTR18126375Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126375Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
KEAP1 Antibody
Product Name Alternative
KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19
UniProt
Q14145
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-EVTPSQHGNRTFS
Target
KEAP1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1) . Recommended concentration: 0.2-0.6μg/mL.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Canine, Porcine, Rat
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_036421.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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