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ZDHHC1 Antibody

CAT: 0013-GTR18126111Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126111Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ZDHHC1 Antibody
Product Name Alternative
ZDHHC1, zinc finger, DHHC-type containing 1, C16orf1, HSU90653, ZNF377, DHHC-domain-containing cysteine-rich protein, zinc finger, DHHC domain containing 1
UniProt
Q8WTX9
Immunogen
The immunogen for this antibody is: C-RNYRLFLHS
Target
ZDHHC1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 20kDa in lysates of cell line HEK293 after 2μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 54.8kDa according to NP_037436.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Human, Mouse, Rat
Tested Applications
ELISA
NCBI Accession Number
NP_037436.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · Q8WTX9

Palmitoyltransferase ZDHHC1

ZDHC1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WTX9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ZDHHC1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.1.225
Processing
—
Secondary accessions
O15461
Protein keywords

Molecular function

AcyltransferaseTransferase

Cellular component

Endoplasmic reticulumEndosomeGolgi apparatusMembrane

PTM

LipoproteinPalmitate

Technical term

Proteomics identificationReference proteome

Domain

TransmembraneTransmembrane helix

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