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CHRNA4 Antibody

CAT: 0013-GTR18126026Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18126026Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CHRNA4 Antibody
Product Name Alternative
CHRNA4, cholinergic receptor, nicotinic, alpha 4, BFNC, EBN, EBN1, NACRA4, cholinergic receptor, nicotinic, alpha 4 subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, neuronal acetylcholine receptor alpha 4 subunit, neuronal nicotinic acetylcholine receptor alpha-4 subunit
UniProt
P43681
Reactivity
Human, Rat
Immunogen
The immunogen for this antibody is: C-HVETRAHAEERLLKK
Target
CHRNA4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ~75kDa confirmed in both human and rat tissues as the glycoprotein by an anonymous customer.This antibody was shown unfit for immune precipitation.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, WB
NCBI Accession Number
NP_000735.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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