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ATP5F1 Antibody

CAT: 0013-GTR18125320Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18125320Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ATP5F1 Antibody
Product Name Alternative
ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, PIG47, ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transport
UniProt
P24539
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-SIQHIQNAIDTE
Target
ATP5F1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research, Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 25kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human Heart lysates (calculated MW of 28.9kDa according to NP_001679.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5μg/mL.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001679.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P24539

ATP synthase peripheral stalk subunit b, mitochondrial

AT5F1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P24539
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ATP5PB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q9BQ68, Q9BRU8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Acetylation

Cellular component

CF(0)MembraneMitochondrionMitochondrion inner membrane

Biological process

Hydrogen ion transportIon transportTransport

Domain

Transit peptide

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