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LPIN3 Antibody

CAT: 0013-GTR18124480Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124480Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LPIN3 Antibody
Product Name Alternative
LPIN3, lipin 3 , RP4-620E11.3, LIPN3L, SMP2, dJ450M14.2, dJ450M14.3, dJ620E11.2 , lipin 3-like
UniProt
Q9BQK8
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KPKQKEDAVATD
Target
LPIN3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_075047.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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