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NQO1 Antibody

CAT: 0013-GTR18124349Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124349Size:0.1 mg
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Description
NQO1 Antibody
Product Name Alternative
NQO1, NAD (P) H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD (P) H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD (P) H menadione oxidoreductase 1, dioxin-inducible, NAD (P) H:Quinone acceptor oxidoreductase type 1, NAD (P) H menadione oxidoreductase 1, dioxin-inducible, NAD (P) H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
UniProt
P15559
Reactivity
Human, Porcine, Rat
Immunogen
The immunogen for this antibody is: C-SIPTDNQIKARK
Target
NQO1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in lysates of Glioblastoma cell line U251 and approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 27.3kDa according to Human NP_001020604.1 and 30.9kDa according to Human NP_000894.1, Rat NP_058696.2 and Pig NP_001153085.1) . Recommended concentration: 0.03-0.3μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. Recommended concentration: 5-10μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_001020604.1, NP_000894.1, NP_001020605.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P15559

NAD(P)H dehydrogenase [quinone] 1

NQO1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15559
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NQO1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.6.5.2
Processing
—
Secondary accessions
B2R5Y9, B4DNM7, B7ZAD1, Q86UK1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cytoplasm

Ligand

FADFlavoproteinNADNADP

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Molecular function

Oxidoreductase

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