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TIA1 Antibody

CAT: 0013-GTR18124007Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124007Size:0.1 mg
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Description
TIA1 Antibody
Product Name Alternative
TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)
UniProt
P31483
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-PKSTYESNTKQ
Target
TIA1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_071505.1, NP_071320.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P31483

Cytotoxic granule associated RNA binding protein TIA1

TIA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P31483
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TIA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q53SS9, Q96B58
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Acetylation

Coding sequence diversity

Alternative splicing

Disease

Amyotrophic lateral sclerosisDisease variantNeurodegeneration

Biological process

ApoptosismRNA processingmRNA splicing

Cellular component

CytoplasmNucleus

Domain

Repeat

Molecular function

RNA-binding

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