Products for Research Use Only

USP28 Antibody

CAT: 0013-GTR18123951Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123951Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
USP28 Antibody
Product Name Alternative
USP28, ubiquitin specific peptidase 28, KIAA1515, ubiquitin specific protease 28
UniProt
Q96RU2
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-SVELKHYIQEDN
Target
USP28
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows acrosomal staining in spermatids. Recommended concentration, 2.5-3.8μg/mL.
Prediction Reactivity
Bovine
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_065937.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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