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HNF1B Antibody

CAT: 0013-GTR18123942Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18123942Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HNF1B Antibody
Product Name Alternative
TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2
UniProt
P35680
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QAYDRQKNPSKEER
Target
HNF1B
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide.Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat, Zebrafish
Tested Applications
ELISA, ICC, WB
NCBI Accession Number
NP_001159395.1, NP_000449.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

UniProtKB · P35680

Hepatocyte nuclear factor 1-beta

HNF1B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35680
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HNF1B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HNF-1-beta, HNF-1B
EC number
—
Processing
—
Secondary accessions
B4DKM3, E0YMJ9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Disease

Diabetes mellitusDisease variant

Domain

Homeobox

Cellular component

Nucleus

PTM

Phosphoprotein

Biological process

TranscriptionTranscription regulation

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