Complement Component C5a Mouse Recombinant Protein

• Description:

  C5a Mouse Recombinant produced in E. coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.

• Synonyms:

  Complement C5; Hemolytic complement; C5; Hc; He; C5a

• Gene Name:

  C5

• UniProt:

  P06684

• Reactivity:

  Mouse

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

• Other Gene Names:

  Complement C5

• Subcellular Location:

  Secreted.