Multiplex ELISA Kit For Mouse Cytokine Panel 1 (6-Plex)

Multiplex ELISA Kit For Mouse Cytokine Panel 1 (6-Plex)

• Description:

  Product Overview

  The Multiplex Mouse Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IFN-γ, IL-1α, IL-1β, IL-6, IL-10, TNFα).

  Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

  Using just 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

  Assay Ranges

  Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
  IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
  IL-1α	Sandwich	610 - 0.84 (pg/mL)	427 (pg/mL)	1.1 (pg/mL)	0.42 (pg/mL)
  IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
  IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
  IL-10	Sandwich	667 - 0.91 (pg/mL)	467 (pg/mL)	1.33 (pg/mL)	0.46 (pg/mL)
  TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

  Kit Components

  Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

  Reagents Include:

  Calibrator: Lyophilized, recombinant antigens in a buffered protein base
  Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
  Substrate A: Liquid, 3.5 mL/vial
  Substrate B: Liquid, 3.5 mL/vial
  Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution
  Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
  Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
  Plate Seals (3): Adhesive strips

• CAS Number: 7732-18-5