Multiplex ELISA Kit For Mouse Cytokine - Inflammation (13-plex)

Multiplex ELISA Kit For Mouse Cytokine - Inflammation (13-plex)

• Description:

  Product Overview

  The Multiplex Mouse Cytokine – Inflammation (13-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 13 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 13 markers in the panel within 5.5 hours.

  The Multiplex Mouse Cytokine – Inflammation (13-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 13 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

  Assay Ranges

  Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
  GMCSF	Sandwich	560 - 0.77 (pg/mL)	392 (pg/mL)	1 (pg/mL)	0.38 (pg/mL)
  IL-1α	Sandwich	610 - 0.84 (pg/mL)	427 (pg/mL)	1.1 (pg/mL)	0.42 (pg/mL)
  IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
  IL-2	Sandwich	360 - 0.49 (pg/mL)	252 (pg/mL)	0.6 (pg/mL)	0.25 (pg/mL)
  IL-3	Sandwich	60 - 0.08 (pg/mL)	42 (pg/mL)	0.1 (pg/mL)	0.04 (pg/mL)
  IL-4	Sandwich	400 - 0.55 (pg/mL)	280 (pg/mL)	0.7 (pg/mL)	0.27 (pg/mL)
  IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
  IL-10	Sandwich	667 - 0.91 (pg/mL)	467 (pg/mL)	1.33 (pg/mL)	0.46 (pg/mL)
  IL-12p70	Sandwich	572 - 0.78 (pg/mL)	400 (pg/mL)	1.01 (pg/mL)	0.39 (pg/mL)
  IL-17A	Sandwich	760 - 1.04 (pg/mL)	532 (pg/mL)	1.4 (pg/mL)	0.52 (pg/mL)
  MIP-1α	Sandwich	1000 - 1.37 (pg/mL)	700 (pg/mL)	1.8 (pg/mL)	0.69
  RANTES	Sandwich	1653 - 2.27 (pg/mL)	1157 (pg/mL)	2.92 (pg/mL)	1.13
  TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29

  Kit Components

  Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format, please contact us for customization.

  Reagents Include:

  Calibrator: Lyophilized, recombinant antigens in a buffered protein base
  Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
  Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
  Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
  Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
  Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
  Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
  Plate Seals (3): Adhesive strips

• CAS Number: 7732-18-5