SEK1/MKK4 (Phospho-Thr261) Colorimetric Cell-Based ELISA Kit

• Description:

  The SEK1/MKK4 (Phospho-Thr261) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SEK1/MKK4 (Phospho-Thr261) protein expression profile in cells. The kit can be used for measuring the relative amounts of SEK1/MKK4 (Phospho-Thr261) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SEK1/MKK4 (Phospho-Thr261) .

• Synonyms:

  Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4;2.7.12.2; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1

• Gene Name:

  MAP2K4

• UniProt:

  P45985

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

• Molecular Weight:

  44288 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Dual specificity mitogen-activated protein kinase kinase 4

• Subcellular Location:

  Cytoplasm. Nucleus.