P27 Kip1 (Phospho-Thr187) Colorimetric Cell-Based ELISA Kit

• Description:

  The p27 Kip1 (Phospho-Thr187) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p27 Kip1 (Phospho-Thr187) protein expression profile in cells. The kit can be used for measuring the relative amounts of p27 Kip1 (Phospho-Thr187) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p27 Kip1 (Phospho-Thr187) .

• Synonyms:

  Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27 ; p27Kip1 ; CDKN1B; KIP1

• Gene Name:

  CDKN1B

• UniProt:

  P46527

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995) . Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A- CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

• Molecular Weight:

  22073 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Cyclin-dependent kinase inhibitor 1B

• Subcellular Location:

  Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) .