JAK3 (Phospho-Tyr785) Colorimetric Cell-Based ELISA Kit

• Description:

  The JAK3 (Phospho-Tyr785) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor JAK3 (Phospho-Tyr785) protein expression profile in cells. The kit can be used for measuring the relative amounts of JAK3 (Phospho-Tyr785) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on JAK3 (Phospho-Tyr785) .

• Synonyms:

  JAK-3; JAK3; Janus kinase 3; L-JAK; Leukocyte janus kinase; kinase Jak3

• Gene Name:

  JAK3

• UniProt:

  P52333

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

• Molecular Weight:

  125099 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Tyrosine-protein kinase JAK3

• Subcellular Location:

  Endomembrane system; Peripheral membrane protein. Cytoplasm.