G3BP-1 (Phospho-Ser232) Colorimetric Cell-Based ELISA Kit

• Description:

  The G3BP-1 (Phospho-Ser232) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor G3BP-1 (Phospho-Ser232) protein expression profile in cells. The kit can be used for measuring the relative amounts of G3BP-1 (Phospho-Ser232) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on G3BP-1 (Phospho-Ser232) .

• Synonyms:

  Ras GTPase-activating protein-binding protein 1; G3BP-1;3.6.4.12;3.6.4.13; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; G3BP1; G3BP

• Gene Name:

  G3BP1

• UniProt:

  Q13283

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Ubiquitous.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

• Molecular Weight:

  52164 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Ras GTPase-activating protein-binding protein 1

• Subcellular Location:

  Cytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity) . Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.