SREBP-1 (Phospho-Ser439) Colorimetric Cell-Based ELISA Kit

• Description:

  The SREBP-1 (Phospho-Ser439) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SREBP-1 (Phospho-Ser439) protein expression profile in cells. The kit can be used for measuring the relative amounts of SREBP-1 (Phospho-Ser439) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SREBP-1 (Phospho-Ser439) .

• Synonyms:

  Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1; SREBF1; BHLHD1, SREBP1

• Gene Name:

  SREBF1

• UniProt:

  P36956

• Reactivity:

  Human, Rat

• Tissue Specificity:

  Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity) . Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') . Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') .

• Molecular Weight:

  121675 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Sterol regulatory element-binding protein 1

• Subcellular Location:

  Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.