WEE1 (Phospho-Ser642) Colorimetric Cell-Based ELISA Kit

• Description:

  The WEE1 (Phospho-Ser642) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor WEE1 (Phospho-Ser642) protein expression profile in cells. The kit can be used for measuring the relative amounts of WEE1 (Phospho-Ser642) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on WEE1 (Phospho-Ser642) .

• Synonyms:

  WEE1hu; Wee1-like protein kinase; kinase WEE1

• Gene Name:

  WEE1

• UniProt:

  P30291

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

• Molecular Weight:

  71597 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Wee1-like protein kinase

• Subcellular Location:

  Nucleus.