IRAK1 (Phospho-Thr100) Colorimetric Cell-Based ELISA Kit

• Description:

  The IRAK1 (Phospho-Thr100) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IRAK1 (Phospho-Thr100) protein expression profile in cells. The kit can be used for measuring the relative amounts of IRAK1 (Phospho-Thr100) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IRAK1 (Phospho-Thr100) .

• Synonyms:

  IL1RAK; IRAK; IRAK-1; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase; kinase IRAK1; mPLK

• Gene Name:

  IRAK1

• UniProt:

  P51617

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

• Molecular Weight:

  76537 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Interleukin-1 receptor-associated kinase 1

• Subcellular Location:

  Cytoplasm. Nucleus. Lipid droplet. Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity) .