Products for Research Use Only
RegisterLogin

IRAK1 Antibody (Phospho-Thr100)

CAT: 0247-OAAF00237-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OAAF00237-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
9007-83-4
Gene Name
Interleukin 1 receptor associated kinase 1
Gene Aliases
Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; pelle; Pelle homolog.
Gene ID
3654
Swiss Prot
P51617
Accession Number
NP_001020413.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr100.
Target
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Reconstitution
-20°C
Molecular Weight
76 kDa
Notes
Formerly GWB-ASB503
Fragment
IgG
Specificity
IRAK1 (Phospho-Thr100) antibody detects endogenous levels of IRAK1 only when phosphorylated at threonine 100.
NCBI Gene Symbol
IRAK1
Host or Source
Rabbit
Protein Name
Interleukin-1 receptor-associated kinase 1
Gene Name URL
IRAK1

Related Products

CatalogName
OKAG01834Phospho-IRAK1 (Thr100) Colorimetric Cell-Based ELISA Kit (OKAG01834)
OKAG01834-2X96WOKAG01834-2X96W - Phospho-IRAK1 (Thr100) Colorimetric Cell-Based ELISA Kit
OASG01817-100ULCREB1 Antibody (Phospho-Thr100)
OASG03948-100ULIRAK1 Antibody (Phospho-Thr209)
OASG03947-100ULIRAK1 Antibody (Phospho-Thr387)
OAAF00447-100UGIRAK1 Antibody (Phospho-Ser376)
OAAF00238-100UGIRAK1 Antibody (Phospho-Thr209)
OASG03950-100ULIRAK1 Antibody (Phospho-Ser376)
OABF01186-100ULIRAK1 Antibody (Phospho-Thr209) (OABF01186-100UL)
OABF01187-100ULIRAK1 Antibody (Phospho-Thr387) (OABF01187-100UL)

Alternative Products

CatalogName
MBS859458-01IRAK1 (Phospho-Thr100) Antibody
AF4441-01Phospho-IRAK1 (Thr100) Antibody
MBS9438635-01IRAK1 (Phospho-Thr100) Conjugated Antibody
70R-36185IRAK1 antibody (Thr100)
TMAC-02208Anti-Phospho-IRAK1 (Thr100) Polyclonal Antibody
abx328498-01Interleukin 1 Receptor Associated Kinase 1 Phospho-Thr100 (IRAK1 pT100) Antibody
AF3921-01Phospho-IRAK1 (Ser376) Antibody
MBS7121434-01Phospho-IRAK1 (T209) Antibody
F54105-0.2MLPhospho-IRAK1 Antibody (S376)
abx012604-01IRAK1 (Phospho-Ser376) Antibody

Popular Products