SP1 (Phospho-Thr453) Colorimetric Cell-Based ELISA Kit

• Description:

  The SP1 (Phospho-Thr453) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SP1 (Phospho-Thr453) protein expression profile in cells. The kit can be used for measuring the relative amounts of SP1 (Phospho-Thr453) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SP1 (Phospho-Thr453) .

• Synonyms:

  TSFP1; Transcription factor SP1; transcription factor Sp1

• Gene Name:

  SP1

• UniProt:

  P08047

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Up-regulated in adenocarcinomas of the stomach (at protein level) . Isoform 3 is ubiquitously expressed at low levels.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation) . Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.

• Molecular Weight:

  80693 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Transcription factor Sp1

• Subcellular Location:

  Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.