FLT3 (Phospho-Tyr599) Colorimetric Cell-Based ELISA Kit

• Description:

  The FLT3 (Phospho-Tyr599) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FLT3 (Phospho-Tyr599) protein expression profile in cells. The kit can be used for measuring the relative amounts of FLT3 (Phospho-Tyr599) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FLT3 (Phospho-Tyr599) .

• Synonyms:

  Tyrosine-protein kinase receptor FLT3; Stem cell tyrosine kinase 1; STK-1; CD135 antigen

• Gene Name:

  FLT3

• UniProt:

  P36888

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level) . Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

• Molecular Weight:

  112903 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Receptor-type tyrosine-protein kinase FLT3

• Subcellular Location:

  Membrane; Single-pass type I membrane protein. Endoplasmic reticulum lumen. Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen. The activated kinase is rapidly targeted for degradation.