GR (Phospho-Ser211) Colorimetric Cell-Based ELISA Kit

• Description:

  The GR (Phospho-Ser211) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor GR (Phospho-Ser211) protein expression profile in cells. The kit can be used for measuring the relative amounts of GR (Phospho-Ser211) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on GR (Phospho-Ser211) .

• Synonyms:

  GCR; GRL; GRL1; NR3C1; glucocorticoid receptor

• Gene Name:

  NR3C1

• UniProt:

  P04150

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991) . In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart (PubMed:10902803) . Isoform Beta:Widely expressed including brain, bone marrow, thymus, spleen, liver, kidney, pancreas, lung, fat, skeletal muscle, heart, placenta and blood leukocytes (PubMed:7769088, PubMed:8621628) . Isoform Alpha-2:Expressed at low level.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Receptor for glucocorticoids (GC) (PubMed:27120390) . Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696) . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514) . Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity) .

• Molecular Weight:

  85659 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Glucocorticoid receptor

• Subcellular Location:

  Isoform Alpha: Cytoplasm. Nucleus. Mitochondrion. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. After ligand activation, translocates from the cytoplasm to the nucleus.