RAD17 (Phospho-Ser645) Colorimetric Cell-Based ELISA Kit

• Description:

  The RAD17 (Phospho-Ser645) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RAD17 (Phospho-Ser645) protein expression profile in cells. The kit can be used for measuring the relative amounts of RAD17 (Phospho-Ser645) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RAD17 (Phospho-Ser645) .

• Synonyms:

  Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog; RAD17; R24L

• Gene Name:

  RAD17

• UniProt:

  O75943

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Overexpressed in various cancer cell lines and in colon carcinoma (at protein level) . Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level) .Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors) .

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

• Molecular Weight:

  77055 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Cell cycle checkpoint protein RAD17

• Subcellular Location:

  Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.