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PIP5K Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1461Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1461Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PIP5K Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PIP5K protein expression profile in cells. The kit can be used for measuring the relative amounts of PIP5K in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PIP5K.
Synonyms
1-phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase;2.7.1.150; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase; PIKFYVE; KIAA0981, PIP5K3
Gene Name
PIKFYVE
UniProt
Q9Y2I7
Reactivity
Human, Mouse
Applications
ELISA
Detection Range
> 5000 cells/well
Function
The PI (3,5) P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P2) . Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Molecular Weight
237136 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
1-phosphatidylinositol 3-phosphate 5-kinase
Subcellular Location
Endosome membrane. Mainly associated with membranes of the late endocytic pathway.

UniProtKB · Q9Y2I7

1-phosphatidylinositol 3-phosphate 5-kinase

FYV1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9Y2I7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PIKFYVE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Phosphatidylinositol 3-phosphate 5-kinase
EC number
2.7.1.150
Processing
—
Secondary accessions
Q08AR7, Q08AR8, Q53ST3, Q53T36, Q8N5H0, Q8NB67
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMetal-bindingNucleotide-bindingZinc

Disease

Corneal dystrophyDisease variant

Cellular component

Cytoplasmic vesicleEndosomeMembrane

Biological process

Host-virus interactionLipid metabolism

Molecular function

KinaseTransferase

Domain

Zinc-finger

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