CD3 zeta Colorimetric Cell-Based ELISA Kit

• Description:

  The CD3 zeta Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CD3 zeta protein expression profile in cells. The kit can be used for measuring the relative amounts of CD3 zeta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CD3 zeta.

• Synonyms:

  CD3-zeta; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain precursor; T3Z; TCRZ

• Gene Name:

  CD247

• UniProt:

  P20963

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641) .

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083) . CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083) . Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity) .

• Molecular Weight:

  18696 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  T-cell surface glycoprotein CD3 zeta chain

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.