Catenin-alpha1 Colorimetric Cell-Based ELISA Kit

• Description:

  The Catenin-alpha1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Catenin-alpha1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Catenin-alpha1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Catenin-alpha1.

• Synonyms:

  102 kDa cadherin-associated protein; Alpha E-catenin; CATNA1; CTN1; Cadherin-associated protein; catenin alpha-1

• Gene Name:

  CTNNA1

• UniProt:

  P35221

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed ubiquitously in normal tissues.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

• Molecular Weight:

  100071 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Catenin alpha-1

• Subcellular Location:

  Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.