Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody

• Background:

  The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR) . The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996) . The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999) . The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996) . Phosphorylation of Ser-1244 has been shown to regulate NMDA receptor channel function (Chen et al., 2006) .

• Description:

  Boster Bio Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody (Catalog # P07769-1) . Tested in WB applications. This antibody reacts with Mouse, Rat.

• Phospho Site:

  Phosphorylated, Ser1244

• Synonyms:

  GluN2C antibody, Glutamate [NMDA] receptor subunit epsilon-3 antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody, Glutamate receptor ionotropic NMDA 2C antibody, Glutamate receptor ionotropic N methyl D aspartate 2C antibody, Grin2c antibody, Grin 2c antibody, GRIN2C antibody, GRIN 2C antibody, N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody, N Methyl D Aspartate Receptor Subtype 2C antibody, N methyl D aspartate receptor subunit 2C antibody, N-methyl D-aspartate receptor subtype 2C antibody, NMDAR2C antibody, NMDE3_HUMAN antibody, NR2C antibody, OTTHUMP00000041930 antibody

• Gene Name:

  GRIN2C

• UniProt:

  Q00961

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1244 of the NR2C subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.

• Applications:

  WB

• Purification:

  Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  WB: 1:1000

• Form:

  Liquid

• Function:

  NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

• Molecular Weight:

  134209 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

• Fragment:

  IgG

• Applications Notes:

  Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser1244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

• Other Gene Names:

  Glutamate receptor ionotropic, NMDA 2C

• Subcellular Location:

  Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

• Prediction Reactivity:

  Bovine, Canine, Human, Primate

• Protein Name:

  Glutamate receptor ionotropic, NMDA 2C