Anti-Phospho-Tau (Ser416) MAPT Antibody

• Background:

  Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985) . Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990) . Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995) . Ser-416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990) .

• Description:

  Boster Bio Anti-Phospho-Tau (Ser416) MAPT Antibody (Catalog # P00097) . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

• Phospho Site:

  Phosphorylated, Ser416

• Synonyms:

  AI413597 antibody, AW045860 antibody, DDPAC antibody, FLJ31424 antibody, FTDP 17 antibody, G protein beta1/gamma2 subunit interacting factor 1 antibody, MAPT antibody, MAPTL antibody, MGC134287 antibody, MGC138549 antibody, MGC156663 antibody, Microtubule associated protein tau antibody, Microtubule associated protein tau isoform 4 antibody, Microtubule-associated protein tau antibody, MSTD antibody, Mtapt antibody, MTBT1 antibody, MTBT2 antibody, Neurofibrillary tangle protein antibody, Paired helical filament tau antibody, Paired helical filament-tau antibody, PHF tau antibody, PHF-tau antibody, PPND antibody, PPP1R103 antibody, Protein phosphatase 1 regulatory subunit 103 antibody, pTau antibody, RNPTAU antibody, TAU antibody, TAU_HUMAN antibody, Tauopathy and respiratory failure included antibody

• Gene Name:

  MAPT

• UniProt:

  P19332

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of rat tau, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

• Applications:

  WB, IHC

• Field of Research:

  Alzheimer's Disease, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Microtubules, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction

• Purification:

  Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Function:

  Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

• Molecular Weight:

  78928 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

• Fragment:

  IgG

• Applications Notes:

  Specific for ~59, 65, 68 kDa tau protein phosphorylated at Ser416. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

• Other Gene Names:

  Microtubule-associated protein tau

• Subcellular Location:

  Cytoplasm, cytosol. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

• Prediction Reactivity:

  Bovine, Canine, Primate

• Protein Name:

  Microtubule-associated protein tau