Anti-Splicing Factor SF3B4 Monoclonal Antibody

• Description:

  Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49; SAP 49; SF3B4; SAP49

• Gene Name:

  SF3B4

• UniProt:

  Q15427

• Host:

  Mouse

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Full length recombinant human SF3B4 expressed in and purified from E. coli

• Clonality:

  Monoclonal

• Applications:

  IF, IHC, WB

• Field of Research:

  DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing, Splicing

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  WB: 1:1,000. IF/ICC and IHC: 1:1,000

• Function:

  Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643) . SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937) . May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well (PubMed:10882114) . Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077) . .

• Molecular Weight:

  44386 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG2b

• Other Gene Names:

  Splicing factor 3B subunit 4

• Subcellular Location:

  Nucleus .