Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

• Background:

  Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

• Description:

  Boster Bio Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody (Catalog # M03238) . Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Dog, Cat, Pig.

• Synonyms:

  Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; BHLHC3, MYF4

• Gene Name:

  MYOG

• UniProt:

  P15173

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Dog, Cat, Pig

• Immunogen:

  Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

• Clonality:

  Monoclonal

• Clone:

  Clone: Clone: F5D

• Applications:

  ELISA, IHC

• Field of Research:

  Mesenchymal Stem Cells, Stem Cells, Transcription

• Purification:

  200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.

• Concentration:

  Purified antibody with BSA and azide at 200ug/ml

• Form:

  Liquid

• Function:

  Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity) . .

• Molecular Weight:

  25037 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

• Fragment:

  IgG1, kappa

• Other Gene Names:

  Myogenin

• Subcellular Location:

  Nuclear