Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband®

• Background:

  Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene. It is mapped to 11p15.5. This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene.

• Description:

  Boster Bio Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® catalog # A07355-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Troponin I, fast skeletal muscle; Troponin I, fast-twitch isoform; TNNI2

• Gene Name:

  Troponin I2, fast skeletal type

• UniProt:

  P48788

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human Troponin I fast skeletal muscle/TNNI2 recombinant protein (Position: D3-S182) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

• Applications:

  ELISA, IF, IHC, WB

• Field of Research:

  Interleukins

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25μg/ml
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
  Immunofluorescence, 5 μg/ml
  ELISA, 0.1-0.5μg/ml
  

• Other Gene Names:

  TNNI2

• Subcellular Location:

  Troponin complex. Cytosol. Nucleus.

• Protein Name:

  Gamma-aminobutyric acid type B receptor subunit 1