Anti-MHC Class II HLA-DPB1 Rabbit Monoclonal Antibody

Anti-MHC Class II HLA-DPB1 Rabbit Monoclonal Antibody

• Description: Boster Bio Anti-MHC Class II HLA-DPB1 Rabbit Monoclonal Antibody catalog # M00487-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
• Synonyms: HLA class II histocompatibility antigen, DP beta 1 chain;HLA class II histocompatibility antigen, DP (W4) beta chain;MHC class II antigen DPB1;HLA-DPB1;HLA-DP1B;
• CAS Number: 9007-83-4
• Gene Name: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt: P04440
• Host: Rabbit
• Reactivity: Human
• Immunogen: A synthesized peptide derived from human MHC Class II
• Clonality: Monoclonal
• Clone: Clone: AAEB-8
• Tissue Specificity: Ubiquitously expressed. .
• Applications: WB,IHC,ICC,IF,IP
• Purification: Affinity-chromatography
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Function: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
• Components: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
• Storage Conditions: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 29159 MW
• Observed Molecular Weight: 52 kDa
• Fragment: Rabbit IgG
• Applications Notes: WB 1:1000-1:5000
  IHC 1:100-1:500
  ICC/IF 1:50-1:200
  IP 1:50
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
• Protein Name: T-cell surface glycoprotein CD4