Anti-AGAP2 Antibody

• Background:

  AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1) -dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.

• Description:

  Boster Bio Anti-AGAP2 Antibody (Catalog # A04771) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

• Synonyms:

  AGAP2, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2, centaurin gamma1, Centg1

• Gene Name:

  AGAP2

• UniProt:

  Q99490

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.

• Clonality:

  Polyclonal

• Applications:

  ELISA, WB

• Field of Research:

  Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

• Purification:

  This protein A purified antibody is directed against human AGAP2 protein. The product was purified from monospecific antiserum by protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with AGAP2 protein from mouse and rat based on 93% and 95% protein:protein homologies, respectively, with the immunizing sequence. Reactivity against homologues from other sources is not known.

• Concentration:

  2.0 mg/mL by UV absorbance at 280 nm

• Form:

  Lyophilized

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

• Shipping Conditions:

  Available

• Storage Conditions:

  Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to AGAP2 protein by western blotting in the appropriate cell lysate or extract.