Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine® Quick ELISA Kit

Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine® Quick ELISA Kit

• Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
• Description: Mouse CXCL5 / LIX / ENA-78 / GCP 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-X-C motif chemokine 5;Cytokine LIX;Small-inducible cytokine B5;GCP-2 (1-78);GCP-2 (9-78);Cxcl5;Scyb5;
• CAS Number: 7732-18-5
• Gene Name: C-X-C motif chemokine 5
• Gene ID: 20311
• UniProt: P50228
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V45-A118
• Applications: ELISA
• Standard: 10 ng/tube
• Sensitivity: <10 pg/ml
• Function: May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2 (1-78) and, more potent, GCP- 2 (9-78) attract neutrophils and are involved in neutrophil activation. .
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Calculated Molecular Weight: 14235 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	35	34	32	34	33	1.08	3.2%
  Sample 2	166	180	162	152	165	10.04	6%
  Sample 3	388	401	390	458	409	28.57	6.9%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse Cxcl5
• Subcellular Location: Secreted.
• Protein Name: C-X-C motif chemokine 5