Mouse CLEC4F ELISA Kit PicoKine®

Mouse CLEC4F ELISA Kit PicoKine®

• Background: C-type lectin domain family 4 member F is a protein that in humans is encoded by the CLEC4F gene. This gene is mapped to 6; 6 C3. CLEC4F is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain that contains an extended stalk region plus one carbohydrate recognition domain. Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation. CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues. CLEC4F preferentially binds galactose and N?acetylgalactosamine in a calcium-dependent manner. Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system.
• Description: Mouse CLEC4F ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CLEC4F in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-type lectin domain family 4 member F; C-type lectin superfamily member 13; C-type lectin 13; CLEC4F; CLECSF13
• CAS Number: 7732-18-5
• Gene Name: C-type lectin domain family 4, member f
• Gene ID: 51811
• UniProt: Q8N1N0
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: R70-G548
• Applications: ELISA
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
• Detection Range: 625 pg/ml - 40,000 pg/ml
• Sensitivity: <300 pg/ml
• : heparin, EDTA or citrate
• Function: Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	963	1121	1018	973	1018	62.56	6.1%
  Sample 2	3961	3775	4002	3504	3810	196.54	5.1%
  Sample 3	15064	17340	17384	14672	16115	1254.77	7.7%

  *number of samples for each test n=16.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse CLEC4F