Mouse AFP ELISA Kit PicoKine®

Mouse AFP ELISA Kit PicoKine®

• Background: alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
• Description: Mouse AFP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Afp in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp
• CAS Number: 7732-18-5
• Gene Name: alpha fetoprotein
• Gene ID: 11576
• UniProt: P02772
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: K19-V605
• Applications: ELISA
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <10 pg/ml
• : heparin or EDTA
• Function: Binds estrogens, fatty acids and metals.
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	787	751	817	732	771	32.75	4.2%
  Sample 2	2378	2440	2261	2414	2373	68.44	2.8%
  Sample 3	9095	10188	9364	8739	9346	534.02	5.7%

  *number of samples for each test n=16.
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse AFP