Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®

• Background: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
• Description: Rat WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Wnt inhibitory factor 1;WIF-1;Wif1;
• CAS Number: 7732-18-5
• Gene Name: Wnt inhibitory factor 1
• Gene ID: 114557
• UniProt: Q6IN38
• Reactivity: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G29-W379
• Applications: ELISA
• Sensitivity: <10 pg/ml
• Function: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 23.4 pg/ml - 1500 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 41585 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	46	51	47	44	47	2.54	5.4%
  Sample 2	179	201	178	187	186	9.2	4.9%
  Sample 3	730	656	670	709	691	29.62	4.2%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant rat WIF1