Human WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®, Carrier-free

• Background:

  Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

• :

  heparin or EDTA

• Description:

  Human WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Human WIF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	43	39	39	39	40	1.73	4.3%
  Sample 2	210	211	236	217	218	10.45	4.7%
  Sample 3	939	920	839	864	890	40.54	4.5%

  *number of samples for each test n=16.

• Synonyms:

  Wnt inhibitory factor 1;WIF-1;WIF1;UNQ191/PRO217;

• Gene Name:

  Wnt inhibitory factor 1

• Gene ID:

  11197

• UniProt:

  Q9Y5W5

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G29-W379

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  23.4 pg/ml - 1500 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  41528 MW

• Specificity:

  Natural and recombinant human WIF1

• Subcellular Location:

  Secreted.

• Protein Name:

  Wnt inhibitory factor 1