Human IL-10/Interleukin-10 ELISA Kit PicoKine®

• Background:

  Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

• Description:

  IL-10/Interleukin-10 ELISA Kit

• Synonyms:

  Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10

• Gene Name:

  Interleukin-10

• UniProt:

  P22301

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: S19-N178

• Tissue Specificity:

  Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

• Applications:

  ELISA

• Field of Research:

  Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <0.5 pg/ml

• Function:

  Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  IL10

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the IL-10 family.

• Protein Name:

  Interleukin-10