Human IL-10/Interleukin-10 ELISA Kit PicoKine®, Carrier-free

• Background:

  Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

• :

  heparin, EDTA or citrate

• Description:

  Human IL-10/Interleukin-10 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	16	17	15	15	15	0.82	5.4%
  Sample 2	53	45	47	48	48	2.94	6.1%
  Sample 3	113	123	106	104	111	7.43	6.6%

  *number of samples for each test n=16.

• Synonyms:

  Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10;

• Gene Name:

  Interleukin-10

• Gene ID:

  3586

• UniProt:

  P22301

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: S19-N178

• Tissue Specificity:

  Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <0.5 pg/ml

• Function:

  Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  20517 MW

• Specificity:

  Natural and recombinant human IL-10

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the IL-10 family.

• Protein Name:

  Interleukin-10