Anti-Mouse CD3E DyLight® 488 conjugated Antibody

• Background:

  CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

• Description:

  Boster Bio Anti-Mouse CD3E DyLight® 488 conjugated Antibody catalog # A02675-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Mouse.

• Synonyms:

  T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; Cd3e

• Gene Name:

  CD3 antigen, epsilon polypeptide

• UniProt:

  P22646

• Host:

  Rabbit

• Reactivity:

  Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113) .

• Clonality:

  Polyclonal

• Conjugation:

  DyLight®488

• Tissue Specificity:

  Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T- cells (spleen) . Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T- cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

• Applications:

  Flow Cytometry

• Field of Research:

  Apoptosis

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Function:

  Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501) . Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

• Storage Conditions:

  At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

• Fragment:

  Rabbit IgG

• Applications Notes:

  6

• Other Gene Names:

  Cd3e

• Subcellular Location:

  Cytoplasm