Anti-CD3 epsilon/CD3E Antibody Picoband®

• Background:

  CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

• Description:

  Boster Bio Anti-CD3 epsilon/CD3E Antibody Picoband® catalog # PB9093. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E

• Gene Name:

  T-cell surface glycoprotein CD3 epsilon chain

• UniProt:

  P07766

• Host:

  Rabbit

• Reactivity:

  Chicken, Human, Mouse, Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207) . Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. .

• Applications:

  IF, IHC, IHC-F, ICC, WB

• Field of Research:

  Adaptive Immunity, Hematopoietic Progenitors, Immunology, Stem Cells, T Cells

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  The CD3 complex mediates signal transduction.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, Chicken, By Heat
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
  Immunocytochemistry , 0.5-1μg/ml, Human
  Immunofluorescence, 2μg/ml, Human, Mouse, Rat
  

• Other Gene Names:

  CD3E

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Contains 1 Ig-like (immunoglobulin-like) domain.

• Protein Name:

  T-cell surface glycoprotein CD3 epsilon chain