Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®

• Description: Boster Bio Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband® catalog # A01981-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Neuronal acetylcholine receptor subunit alpha-3; CHRNA3; NACHRA3
• CAS Number: 9007-83-4
• Gene Name: cholinergic receptor nicotinic alpha 3 subunit
• Gene ID: 1136
• UniProt: P32297
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively.
• Clonality: Polyclonal
• Applications: Flow Cytometry,WB
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
• References & Citations: 1. "Entrez Gene: CHRNA3 cholinergic receptor, nicotinic, alpha 3". 2. Eng CM, Kozak CA, Beaudet AL, Zoghbi HY (Apr 1991). "Mapping of multiple subunits of the neuronal nicotinic acetylcholine receptor to chromosome 15 in man and chromosome 9 in mouse". Genomics. 9 (2): 278–82. 3. Mihovilovic M, Roses AD (1991). "Expression of mRNAs in human thymus coding for the alpha 3 subunit of a neuronal acetylcholine receptor.". Exp. Neurol. 111 (2): 175–80.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Observed Molecular Weight: 60 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot,0.1-0.5μg/ml
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human