Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband®

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband®

• Background: CHRNA, also termed ACHRA, is mapped on 2q24-q32.This gene encodes the alpha subunit of the muscle acetylcholine receptor, which is the main target of pathogenic autoantibodies in autoimmune myasthenia gravis. The protein-coding sequence of the human alpha subu gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule.
• Description: Boster Bio Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody catalog # PA1203. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Acetylcholine receptor subunit alpha;CHRNA1;ACHRA, CHNRA;
• CAS Number: 9007-83-4
• Gene Name: Acetylcholine receptor subunit alpha
• UniProt: P02708
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1, identical to the related rat sequence, different from the related mouse sequence by one amino acid.
• Clonality: Polyclonal
• Applications: WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
• References & Citations: 1. Giraud, M.; Taubert, R.; Vandiedonck, C.; Ke, X.; Levi-Strauss, M.; Pagani, F.; Baralle, F. E.; Eymard, B.; Tranchant, C.; Gajdos, P.; Vincent, A.; Willcox, N.; Beeson, D.; Kyewski, B.; Garchon, H.-J. : An IRF8-binding promoter variant and AIRE control CHRNA1 promiscuous expression in thymus. Nature 448: 934-937, 2007. 2. Noda, M.; Furutani, Y.; Takahashi, H.; Toyosato, M.; Tanabe, T.; Shimizu, S.; Kikyotani, S.; Kayano, T.; Hirose, T.; Inayama, S.; Numa, S. : Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor. Nature 305: 818-823, 1983.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 54546 MW
• Observed Molecular Weight: 69 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse