Anti-ADAM10/ADAM10 Antibody Picoband®

Anti-ADAM10/ADAM10 Antibody Picoband®

• Background: ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also know as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.
• Description: Boster Bio Anti-ADAM10/ADAM10 Antibody catalog # PA1535. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Disintegrin and metalloproteinase domain-containing protein 10;ADAM 10;3.4.24.81;CDw156;Kuzbanian protein homolog;Mammalian disintegrin-metalloprotease;CD156c;ADAM10;KUZ, MADM;
• CAS Number: 9007-83-4
• Gene Name: Disintegrin and metalloproteinase domain-containing protein 10
• UniProt: O14672
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10.
• Clonality: Polyclonal
• Applications: WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling. .
• References & Citations: 1. Wolfsberg, T. G., Primakoff, P., Myles, D. G., White, J. M.ADAM, a novel family of membrane proteins containing a disintegrin and metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions.J. Cell Biol. 131: 275-278, 1995. 2. Yamazaki, K., Mizui, Y., Tanaka, I.Radiation hybrid mapping of human ADAM10 gene to chromosome 15.Genomics 45: 457-459, 1997. 3. Lunn, C. A., Fan, X., Dalie, B., Miller, K., Zavodny, P. J., Narula, S. K., Lundell, D.Purification of ADAM 10 from bovine spleen as a TNFalpha convertase.FEBS Lett. 400: 333-335, 1997.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 84142 MW
• Observed Molecular Weight: 31 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human